Protein Expression Products

VE-BEVS Complete Kit 30033 (VE-CL-03, pACVE.03)

30033

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VE-BEVS Complete Kit 30033 contains Product 10030 Insect Cell Line (VE-CL-03) and Product 20030 Transfer Vector (pAcVE.03).

• VE-BEVS Insect Cell Line 10030 (1x10⁷ cells; frozen) (VE-CL-03) General enhancement of most recombinant proteins

• VE-BEVS Transfer Vector 20030 (10 µg) (pAcVE.03) Co-expression of 𝘷𝘢𝘯𝘬𝘺𝘳𝘪𝘯 gene for increased protein production; HBM signal sequence for direct secretion and C-terminal 6xHis-tag for ease of purification of protein of interest. Vankyrin-Enhanced (VE) baculovirus transfer vector designed for high levels of recombinant gene expression under the control of the Autographa californica nucleopolyhedro virus (AcMNPV) polyhedrin promoter. 

Advantages

VE-BEVS Insect Cell Line 10030

  • Engineered monoclonal Sf9 cell line stably expresses a vankyrin gene
  • Mycoplasma free
  • Up to 14-fold increase in protein yield as compared to parental Sf9 cells
  • Prolonged longevity of cells after infection with a baculovirus compared to parental Sf9 cells
  • Increased baculovirus titer
  • Compatible with all conventional BEVS
  • Reduced cost and labor
  • Expression of recombinant protein may need to be optimized by testing different time points and MOIs.

Transfer Vector 20030 (pAcVE.03)

  • This baculovirus transfer vector integrates the co-expression of a vankyrin gene from the p10 promoter for the increased production of gene of interest from the polyhedrin promoter with:

    • Honey bee melittin signal sequence for direct secretion and
    • Optional C-terminal 6x his-tag for ease of purification

      Documents

      VE-BEVS Cell Line Instructions [PDF]

      Transfer Vector pAcVE.03 [PDF]

      pAcVE.03 Instructions [PDF]

      VE-BEVS Cell Line FAQs [PDF]

      VE-BEVS Licensing

      Contract service for introducing the vankyrin gene into a custom insect cell line is available upon request.

      Patent information:
      VE-BEVS Cell Line United States Patent 7,842,493 [PDF]
      VE-BEVS Transfer Vector United States Patent 7,629,160 [PDF]

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