Protein Expression Products

VE-BEVS Insect Cell Line 10030 (1x10⁷ cells; frozen) (VE-CL-03) General enhancement of most recombinant proteins

10030

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ParaTechs’ Vankyrin-Enhanced Baculovirus Expression Vector System or VE-BEVS technology enhances protein expression from the BEVS while reducing the cost of labor and materials. Vankyrin-Enhanced Insect Cells (VE cells) are transgenic insect Spodoptera frugiperda (Sf9) cells that have been engineered to stably express the Campoletis sonorensis ichnovirus P-vank-1 protein (Steele et al., 2017; Fath-Goodin et al., 2006; Kroemer and Webb, 2006). Sf9-derived VE-CL-03 cell line suitable for the enhanced expression of proteins of unknown toxicity and stability or for general enhancement of most recombinant proteins expressed in conventional recombinant BEVS viruses. The cells are sold in batches of 1×10⁷/ml cells in serum-free medium and need to be stored in liquid nitrogen. Please note this product ships on dry ice so additional shipping charges may apply. This product is intended for research purposes only.

Advantages

  • Engineered monoclonal Sf9 cell line stably expresses a vankyrin gene
  • Mycoplasma free
  • Up to 14-fold increase in protein yield as compared to parental Sf9 cells
  • Prolonged longevity of cells after infection with a baculovirus compared to parental Sf9 cells
  • Increased baculovirus titer
  • Compatible with all conventional BEVS
  • Further enhancement can be achieved in combination with ParaTechs’ pAcVE transfer vectors (Product No. 20010, 20020 or 20030).  View our VE-BEVS Insect Cell Line 10030 Complete Kits 3003130032 and 30033 for more information.
  • Reduced cost and labor
  • Expression of recombinant protein may need to be optimized by testing different time points and MOIs.

    Documents

    VE-BEVS Cell Line Instructions [PDF]

    VE-BEVS Cell Line FAQs [PDF]

    VE-BEVS Licensing

    Contract service for introducing the vankyrin gene into a custom insect cell line is available upon request.

    Patent information:
    VE-BEVS Cell Line United States Patent 7,842,493 [PDF]
    VE-BEVS Transfer Vector United States Patent 7,629,160 [PDF]

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