mNSET™ (Non-Surgical Embryo & Sperm Transfer) Device for Mice 60010

60010 (10 devices per box)

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10 Devices/Box

ParaTechs’ mNSET™ (Non-Surgical Embryo and Sperm Transfer) Device for Mice 60010 revolutionizes mouse uterine embryo transfer by reducing cost and eliminates the need for animal anesthesia and recovery normally required for conventional surgical procedures. Compare this methodology with the time, expense, and training required to accomplish the same task with other methods and you will discover that the NSET procedure is humane, quick and cost effective.

The mNSET Device for Mice 60010 is manufactured in the USA by an FDA Registered Medical Device Manufacturer and ISO 13485:2003 Registered Company and is EtO (Ethylene Oxide) sterilization processed. Patent Information: Non-Surgical Embryo Transfer Method and Apparatus, United States Patent 9,615,903. [PDF]

 

Click on the Merit Ribbon to view our Award Winning Poster during D5 AALAS 2014, Stone B. (2014) “A Non-surgical Uterine Transfer Technique for Mouse Embryos after Cryopreservation, In Vitro Fertilization, ES-cell Injection, and Sperm during Artificial Insemination.”

 

mNSET 60010 Videos

  • mNSET 60010 Full Procedure Demonstration Video: 

  • mNSET 60010 Quick Procedure Only Video: 

mNSET 60010 Webinar

Watch the webinar presented at Lab Animal Sciences 2014 by Dr. Barbara Stone, Director of NSET Technology, ParaTechs Corporation, C.E. Credits: CE

ABSTRACT: Non-surgical transfer techniques for mouse embryos and sperm provide animal welfare benefits for assisted reproduction of mice. While surgical embryo transfer (ET) is an effective method for transfer of embryos into the uterine horn of mice, surgery is expensive, time consuming, and requires technical expertise. Surgery is also a stressful procedure for the mouse, which has to be anesthetized and treated with an analgesic. We have developed a simple, brief procedure for ET using a non-surgical embryo transfer (NSET) device. Once embryos are loaded into the NSET device, the tapered NSET catheter passes through the vagina and traverses the cervix to deposit the embryos into the uterine horn of a recipient mouse. Since the NSET procedure does not require sedation, opening of the inner body cavity, or use of an analgesic, this procedure is less stressful for the mouse than surgery. In proof, our results show that when pseudopregnant mice are monitored by electrocardiography, the non-surgical procedure does not affect heart rate. The NSET procedure also has no effect on the level of the stress biomarker fecal corticosterone measured by ELISA analysis. However, surgery or anesthesia does lower heart rate and increases levels of fecal corticosterone. Additional studies show that the NSET procedure is effective for transfer of mouse embryos for the purposes of rederivation, cryorecovery, and transgenic research. Embryos can be successfully transferred with the NSET technique after cryopreservation, in vitro fertilization (IVF), or embryonic stem (ES) cell injection as an alternative to traditional surgical embryo transfer. The NSET device has also successfully transferred mouse sperm for artificial insemination. Therefore, the NSET technique is an example of a 3Rs refinement, an alternative to a surgical procedure, which will lead the way for improved assisted reproductive techniques in mice.

 

Uses

Mouse Embryo Transfer:

  • Embryo transfer after DNA microinjection
  • ES cell chimeric blastocyst transfer
  • Cryopreserved embryo transfer
  • Embryo transfer after in vitro fertilization
  • Embryo transfer for rederivation

Mouse Sperm Transfer for Artificial Insemination (AI):

  • Fresh sperm
  • Cryopreserved sperm

Pathogen Transfer:

  • Chlamydia trachomatis

Material Transfer:

  • Sepharose beads
  • Pharmaceuticals

Advantages

  • Eliminates the pain and distress of surgery
  • No anesthesia required
  • Eliminates need for post-surgical monitoring of animals
  • Reduces regulatory burden by eliminating need to justify survival surgery
  • Eliminates surgical instruments and time-consuming pre- and post-surgical processes
  • Greatly reduces time required to become proficient in embryo transfer or artificial insemination (AI)
  • Reduces costs of embryo transfer
  • Reduces costs of AI
  • Easy procedure
  • Convenient
  • Sterile and ready to use
  • 3Rs implementation (Reduce & Refine)

Documents

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